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BackgroundAdipose-derived stem cells (ASCs) are being increasingly recognized for their potential to promote tissue regeneration and wound healing. These effects appear to be partly mediated by paracrine signaling pathways, and are enhanced during hypoxia. Mass spectrometry (MS) is a valuable tool for proteomic profiling of cultured ASCs, which may help to reveal the identity of the factors secreted by the cells under different conditions. However, serum starvation which is essentially required to obtain samples compatible with secretome analysis by MS can have a significant influence on ASCs. Here, we present a novel and optimized culturing approach based on the use of a clinically relevant serum-free formulation, which was used to assess the effects of hypoxia on the ASC proteomic profile.
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The Patriot soundtrack from 2000, composed by John Williams. Released by Hollywood Records in 2000 (HR-62258-2) containing music from The Patriot (2000).
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Street Fighter X Tekken PC merupakan sebuah permainan dengan genre duel pertarungan yang dikembangkan oleh Capcom. Game ini pertama kali dirilis pada konsol Playstation dan Xbox 360 kemudian dilanjutkan versi PC pada May 2012. System Requirements OS: Windows XP CPU: Intel Dual Core @ 1.8 GHz / AMD Athlon II X2 Video Card: nVidia GF6600. View Article.
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